In almost all search engines, users must limit their search space to a canonical database (and handful of PTMs) due to time constraints and q value considerations, but this typically does not reflect the individual genetic variations of the organism being studied. Bolt is a new cloud-based search engine that can search more than 6,000,000 protein sequences (canonical, isoform, mutations, contaminants, non-canonical) with more than 400 post-translation modifications and N-terminal and C-terminal partial tryptic search in minutes on a standard configuration laptop. Along with increases in speed, Bolt provides an additional benefit of improvement in high-confidence identifications.
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